Although biodiversity is at the core of global biogeochemical cycles and homeostasis of the Earth system, the complexity and non-unified nature of biodiversity as a concept has hindered the development of simple standards to measure it holistically at local to global scales. We propose that metabarcoding with the universal primers, 515F-Y-926R, provides a scalable, feasible and objective solution for tree-of-life scale biodiversity assessments. Recognizing the value of this approach, several projects within BIOcean5D are already leveraging the 515F-Y-926R primers for generating amplicon data. The aim of this workshop is to a) demonstrate the efficacy of using the 515F-Y-926R primers to measure biodiversity, and b) present a standardised workflow for processing and analysing amplicon data generated using the primers.
Staff and facilitators:
- Taylor Priest - NOMIS-ETH Fellow, Institute of Microbiology and Centre of Origins and Prevalence of Life, ETH Zurich.
- Nicolas Henry - Research Engineer, ABiMS, Station Biologique de Roscoff, Roscoff, Bretagne, FR
Prerequisites
Audience
The workshop is targeted towards all members of the BIOcean5D who will either directly or indirectly use SSU rRNA gene amplicon sequencing as a method to achieve their research objectives.
Note: Registration limited to B5D partners only (including ECRs)
Grant opportunities
This workshop is jointly organised by Institute of Microbiology and Centre of Origins and Prevalence of Life, ETH Zurich,
Station Biologique de Roscoff and EMBRC-BE/UGent for the BIOcean5D (Marine biodiversity assessment and prediction across spatial, temporal and human scales) Project, and funded by the European Commission under the Grant Agreement number 101059915.
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