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Methods for Implementation of Surveillance Procedures for Listed Fish Diseases



Course format On-site
Date 2020-10-05 - 2020-10-11

General course objectives

The course aims to provide the participants with knowledge on the most used methods for diagnosis of important fish viruses.

Learning outcomes

A student who has met the objectives of the course will be able to:

  • Sample and process material for diagnostic purpose
  • Maintain, cultivate, inoculate and read most used cell lines (BF-2, EPC, CCB and ASK) for fish disease diagnostic purposes
  • Prepare cell cultures for different purposes, e.g. diagnosis, IFAT and virus titration
  • Inoculate and sub-cultivate diagnostic samples
  • Read diagnostic trays
  • Titrate virus
  • Apply Real-Time PCR for surveillance purposes
  • Genotype important viral isolates by sequencing and blasting
  • Assess pitfalls and errors in test performances and designs.


ISCED Categories

Machinery and operators